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Different Plasmodium falciparum Recombinant MSP1 19 Antigens Differ in Their Capacities to Stimulate In Vitro Peripheral Blood T Lymphocytes in Individuals from Various Endemic Areas
Author(s) -
GARRAUD O.,
DIOUF A.,
NGUER C. M.,
DIEYE A.,
LONGACRE S.,
KASLOW D. C.,
HOLDER A. A.,
TALL A.,
MOLEZ J. F.,
PERRAUT R.,
MERCEREAUPUIJALON O.
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00511.x
Subject(s) - plasmodium falciparum , biology , peripheral blood mononuclear cell , antigen , immune system , population , recombinant dna , malaria vaccine , lymphocyte , immunology , t cell , virology , in vitro , microbiology and biotechnology , malaria , medicine , gene , biochemistry , environmental health
This study reports on T‐cell proliferative responses to the 19‐kDa C‐terminal domain of the Plasmodium falciparum merozoite surface protein (MSP1 19 ). Three different recombinant proteins were used: an Escherichia coli product expressing the first EGF‐like domain and Saccharomyces cerevisiae and baculovirus/insect‐cell‐produced proteins containing both EGF‐like domains, the latter protein being produced with or without N ‐glycosylation. Cell donors were P. falciparum ‐immune adults with no recent history of clinical malaria and recruited from three Senegalese settings with different epidemiological parasite transmission. Each mononuclear‐blood‐cell preparation was stimulated with a range of concentrations of the three proteins. Most subjects’ mononuclear cells were reactive to at least one protein, but significant differences in lymphoproliferation were seen between the settings and within individual cultures depending on the protein source and concentration. Importantly, lymphoproliferation indices correlated inversely with the intensity of P. falciparum malaria transmission. When purified T lymphocytes were cultured in the presence of MSP1 19 plus autologous monocytes, B lymphocytes or a proposed CD1 + dendritic‐cell population as costimulatory cells, significant differences were observed depending on the individual's previous exposure to parasites. This study shows that the stimulation of lymphocyte proliferation in vitro with MSP1 19 depends on several factors, including epidemiological conditions and protein preparations.