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Efficient Presentation of House Dust Mite Allergen Der p 2 by Monocyte‐Derived Dendritic Cells and the Role of β2 Integrins
Author(s) -
S.-S. J. Sung,
Ernesto Akio Taketomi,
Anderson D. Smith,
Thomas A.E. Platts-Mills,
Shu Man Fu
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00462.x
Subject(s) - house dust mite , immunology , allergen , monocyte , integrin , dendritic cell , biology , medicine , immune system , allergy , cell , genetics
Monocyte‐derived dendritic cells (MDDC) are potent antigen‐presenting cells (APC). The APC functions of MDDC in allergy were examined. MDDC presentation of the major house dust mite allergen Der p 2 resulted in 4–12‐fold higher T‐cell proliferation and markedly higher IFN‐γ and IL‐5 production than PBMC cultures. Comparable T‐cell proliferation was obtained with 10‐fold fewer MDDC than purified monocytes. MDDC cultured from adherent cells, or CD14 + , CD11b + or Percoll‐purified monocytes were comparable in presenting soluble Ag, and in stimulating allogeneic MLR. Importantly, MDDC presentation of Der p 2 resulted in both Th1 and Th2 stimulation, although MDDC are known to produce high levels of IL‐12 and stimulate biased Th1 responses. The basis for the potent APC function of MDDC was further examined. MDDC were found to be highly phagocytic. Immunoprecipitation studies showed markedly elevated ICAM‐1 expression but > 10‐fold reduction in LFA‐1 expression on MDDC compared with monocytes. Monoclonal antibody (MoAb)‐blocking experiments showed that ICAM–LFA‐1 interaction was essential for MDDC stimulation of Der p 2‐specific T‐cell proliferative responses. These results show that the use of MDDC as APC provides a simple, sensitive and versatile method for detecting T‐cell responses to allergens and that the strong phagocytic capability and the increased ICAM‐1 expression of MDDC contribute significantly to their Ag presentation potency.