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Antibodies against Macaque Monoclonal Immunoglobulin G in Rheumatoid Arthritis
Author(s) -
Françoise Fortenfant,
F Garrette-Gaston,
F Oksman,
B. Fournié,
Antoine Blancher
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00450.x
Subject(s) - rheumatoid arthritis , agglutination (biology) , antibody , macaque , latex fixation test , monoclonal antibody , medicine , immunology , rheumatoid factor , monoclonal , immunoglobulin g , biology , paleontology
The presence of rheumatoid factors (RF) in the serum of rheumatoid arthritis (RA) patients is commonly evidenced by agglutination tests: the Waaler–Rose assay, based on the use of human red blood cells (RBCs) coated with rabbit anti‐RBC antibodies, and the latex test, which uses latex particles coated with denatured human immunoglobulin G (IgG). The aim of the present study was to characterize the RF able to agglutinate human RBCs coated with macaque antihuman RBC IgG antibodies secreted from macaque–mouse heterohybridomas (two from rhesus monkey and one from crab‐eating macaque). Human RBCs coated with macaque monoclonal antibodies (MacMoAbs) were used for agglutination tests and these were carried out in parallel with standard tests (Waaler–Rose and latex agglutination tests) on sera from 82 RA patients, 86 patients with other forms of inflammatory chronic arthritis and 47 healthy human subjects. MacMoAb‐coated RBCs identified RF in the sera of 66% patients with RA. By contrast, the frequency of positive sera in other inflammatory diseases was 5% and all 47 healthy controls were negative. Antimacaque IgG antibodies were found to be more specific for RF than standard tests, in the sera of patients with RA.