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Establishment and Characterization of a Human T‐Lymphocyte Cell Line Immortalized by SV40 and with Abnormal Expression of TCR/CD3
Author(s) -
Martine Chatelut,
Sylvie Caspar-Bauguil,
Jean Tkaczuk,
Laeticia Alibaud,
M T Pieraggi,
Samira Roudani,
Nathalie Vacaresse,
Jean Feunteun,
Patrick Laharrague,
Éliane Duchayne,
Cécile Demur,
Mark Vincent,
Jean-Claude Thiers,
Robert Salvayre,
Thierry Levade
Publication year - 1998
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1998.00447.x
Subject(s) - biology , t cell receptor , cd3 , microbiology and biotechnology , major histocompatibility complex , il 2 receptor , cell culture , t cell , immortalised cell line , t lymphocyte , jurkat cells , antigen , lymphocyte , virology , cd8 , immunology , immune system , genetics
Human lymphocytes derived from the peripheral blood of a healthy woman were transfected with a plasmid carrying the simian virus 40 (SV40) large T antigen. The successfully transformed cells contained SV40 large T DNA and were negative for Epstein–Barr virus (EBV) and human T‐cell leukaemia virus (HTLV)‐1 genomes. The immortalized cell line was assigned to the T‐lymphocyte lineage on the basis of morphological, immunological and cytochemical criteria. While the cells expressed CD1a and CD4 at the cell surface, the CD3 complex was solely intracytoplasmic. Immunoprecipitation studies indicated that these cells lacked T‐cell receptor (TCR) α‐chains but not β‐chains. They were negative for activation markers such as CD25, CD69 and major histocompatibility (MHC) class II molecules. In addition, the transformed cells exhibited a complete growth independency towards interleukin‐2 (IL‐2). However, after phorbol ester stimulation, CD25 and CD69 markers were expressed and IL‐2 was secreted. This new human immortalized T‐lymphocytic cell line, which is cell‐surface TCR/CD3‐negative, may be useful as an in vitro model for studying TCR/CD3 assembly, expression and signal transduction.