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Immunological Characterization of α Antigen of Mycobacterium kansasii : B‐Cell Epitope Mapping
Author(s) -
Mariko Naito,
Naoya Ohara,
Sohkichi Matsumoto,
Takeshi Yamada
Publication year - 1998
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1998.00368.x
Subject(s) - epitope , mycobacterium kansasii , mycobacterium tuberculosis , antigen , amino acid , recombinant dna , epitope mapping , peptide sequence , biology , microbiology and biotechnology , virology , tuberculosis , biochemistry , immunology , medicine , gene , pathology
Mapping of B‐cell epitopes on α antigen of Mycobacterium kansasii (K‐α) was carried out by using recombinant truncated K‐α fusion peptides. We observed that two immunodominant B‐cell epitopes (amino acids 222–268 and 267–306) and one minor epitope (amino acid 249–286) were located in the C‐terminal region of K‐α. The other three minor B‐cell epitopes were mapped in N‐terminal (amino acids 80–98 and 99–166) and central (amino acid 174–204) regions of K‐α. All defined epitopes were common to Mycobacterium tuberculosis and M. kansasii . Besides these common epitopes, a region in K‐α (amino acid 290–319) revealed different reactivities between antibodies against K‐α and α antigen of M. tuberculosis . These findings may provide a basis for development of serodiagnosis that can distinguish between M. kansasii and M. tuberculosis infections.