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Susceptibility and Resistance to Leishmania amazonensis in H‐2 q Syngeneic High and Low Antibody Responder Mice (Biozzi Mice)
Author(s) -
) Lima,
Anne Puel,
Decreusefond,
Bouthillier,
Mevel,
Abrahamsohn,
; Mouton
Publication year - 1998
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1998.00364.x
Subject(s) - biology , cytokine , major histocompatibility complex , immune system , antigen , leishmania major , microbiology and biotechnology , interleukin 4 , antibody , leishmania , t cell , intracellular , immunology , parasite hosting , world wide web , computer science
H‐2 syngeneic H and L (Biozzi) mice provide a model to study Leishmania infections in which polar resistant and susceptible phenotypes are independent from H‐2 differences. High‐Ab‐responder (H) and low‐Ab‐responder (L) mice syngeneic at the H‐2 locus (H‐2 q ) were, respectively, susceptible and highly resistant to Leishmania amazonensis infection. L‐mice resistance was associated with high IFN‐γ and transient IL‐4 production by lymph node (LN) cells, in contrast with sustained IL‐4 and decreasing IFN‐γ production by susceptible H mice. IL‐12 production could be detected only in LN from resistant mice. The cytokine production pattern was consistent with preferential progression to a Th1‐type response in resistant L‐mice, and to a Th2‐type response in susceptible H‐mice. We also investigated whether this shift towards Th1‐ or Th2‐type cytokine responses was dependent upon H or L antigen presenting cells' (APC) intrinsic ability to preferentially stimulate either T‐cell subset. To this end, LN‐derived T‐cell lines were grown from 12‐day infected mice, when both strains produced IFN‐γ and IL‐4. L‐derived T‐cell lines developed a Th2 cytokine pattern whereas H‐derived T‐cell lines produced IFN‐γ, IL‐4 and IL‐10 whatever the APC origin (H or L) used for their derivation. This work constitutes the first characterization of cellular immune responses to the intracellular parasite, L. amazonensis in H‐2 syngeneic mice, an infection model in which polar resistant and susceptible phenotypes are determined by non‐MHC genes.