Premium
Antibody‐Dependent Cell‐Mediated Cytotoxicity to Gliadin‐Coated Cells with Sera from Children with Coeliac Disease
Author(s) -
Robert Saalman,
Agnes E. Wold,
U. Dahlgren,
S. P. Fällström,
Lars Å. Hanson,
S. Ahlstedt
Publication year - 1998
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1998.00244.x
Subject(s) - antibody dependent cell mediated cytotoxicity , gliadin , coeliac disease , immunology , antibody , effector , cytotoxic t cell , cytotoxicity , monocyte , medicine , disease , biology , gluten , in vitro , pathology , biochemistry , monoclonal antibody
Antibody‐dependent cell‐mediated cytotoxicity (ADCC) has been suggested as a contributing immunological mechanism in the disease process of coeliac disease. In the present study, sera from coeliac children were examined for their capacity to mediate ADCC against gliadin‐coated target cells. The ADCC‐mediating efficacy of sera were tested using monocytes from healthy adults as effector cells and gliadin‐coated erythrocytes from the same donor as targets. Using monocytes as effector cells, sera from children with active coeliac disease (untreated or challenged), demonstrated significantly higher ADCC‐mediating capacity than sera from healthy and disease references as well as children with treated coeliac disease. A positive correlation was found between the ADCC‐mediating capacity and serum IgG as well as IgA anti‐gliadin antibody levels. The results suggest that an antibody‐dependent monocyte/macrophage‐induced cytotoxic reaction might be involved in the disease process of coeliac disease.