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Lack of Correlation Between Membrane CD30 Expression and Cytokine Secretion Pattern in Allergen‐Primed Naive Cord Blood T‐Cell Lines and Clones
Author(s) -
SPINOZZI F.,
AGEA E.,
PIATTONI S.,
FALINI B.,
GRIGNANI F.,
BERTOTTO A.
Publication year - 1997
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1997.d01-416.x
Subject(s) - immunology , il 2 receptor , antigen , biology , cord blood , cytokine , t cell , priming (agriculture) , interleukin 21 , microbiology and biotechnology , cd8 , immune system , botany , germination
Various surface molecules are expressed by activated T cells. Among them, the CD30 antigen has been proposed as a reproducible marker that identifies a subset of differentiated and/or activated T lymphocytes that produce T helper (Th)‐2‐type cytokines, i.e. interleukin (IL)‐4 and IL‐5. However, because CD30 has mainly been detected on established T‐cell clones, it is still unclear whether a priming allergen and/or cytokine can induce its membrane expression on naive T cells, perhaps in parallel with the up‐regulation of other relevant activation markers, such as CD25, HLA‐DR and L‐selectin. It is also unknown whether proper allergen stimulation affects the cytokine secretion pattern by CD30 + T‐cell clones derived from antigen‐unprimed (naive) T lymphocytes. More information on these questions was sought by adopting a model that used cord blood as a source of virgin T cells and exposing them to native cypress allergen or cytokine (IL‐2 or IL‐4) stimulation, as well as to conventional polyclonal activators such as PHA or anti‐CD3. Peripheral blood MC from four adult cypress‐sensitive patients was also assayed and used as controls for all culture experiments. Freshly isolated cord and adult T cells did not express the CD30 antigen on their membrane. Many of the stimulating agents tested were able to up‐regulate the expression of CD30. However, despite high expression of this molecule, cloned allergen‐specific cord CD4 + T lymphocytes were unable to produce IFN‐γ and/or IL‐4. In contrast, they retained the capability to produce IL‐2. Thus, expression of the CD30 antigen on virgin T cells does not correlate with a polarized model of T helper (Th)‐1 or Th‐2 cytokine‐producing cells, suggesting that these types of lymphokine‐secreting lymphocytes are not a paradigmatic example of T‐cell subpopulations that display stable phenotypical features.