Premium
ICAM‐2 Provides a Costimulatory Signal for T Cell Stimulation by Allogeneic Class II MHC
Author(s) -
CARPENITO C.,
PYSZNIAK A. M.,
TAKEI F.
Publication year - 1997
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1997.d01-391.x
Subject(s) - antigen presenting cell , transfection , antigen presentation , t cell , mhc class ii , cd40 , antigen , icam 1 , spleen , microbiology and biotechnology , cytotoxic t cell , biology , cd1 , immunology , immune system , cell culture , cell adhesion molecule , in vitro , biochemistry , genetics
To examine the functional role of intercellular adhesion molecule (ICAM)‐2 (CD102) in antigen presentation to T cells, the I‐E d ‐transfected murine fibroblastic L cell line RT10.3 (H‐2 k ) was transfected with murine ICAM‐1 or ICAM‐2 and tested for their abilities to stimulate C3H/He (H‐2 k ) splenic T cells. The expression of ICAM‐1 or ICAM‐2 on RT10.3 cells significantly increased the stimulation of T cells in an LFA‐1 (CD11a/CD18)‐dependent manner as determined by thymidine incorporation. This enhanced T cell response was also observed when combinations of untransfected RT10.3 cells and ICAM‐1‐ or ICAM‐2‐transfected L cells were used as stimulators, indicating that ICAM‐1 and ICAM‐2 deliver a costimulatory signal instead of merely enhanced T cell adhesion to antigen presenting cells. The T cells stimulated with ICAM‐transfected RT10.3 in the primary response vigorously responded to BALB/c (H‐2 d ) spleen cells in a secondary allogeneic stimulation. In contrast, T cells stimulated with untransfected RT10.3 in the primary response did not respond to BALB/c spleen cells in the secondary response. Significant secondary responses to a third party stimulator, C57BL/6 spleen cells (H‐2 b ), were observed regardless of ICAM expression on RT10.3 cells in the primary stimulation. These results indicate that ICAM‐2 as well as ICAM‐1 not only enhance antigen presentation mediated by allogeneic class II MHC but also provide a costimulatory signal to T cells. This costimulatory signal may be important in the aversion of an anergic state.