Premium
Contrary Roles of IL‐4 and IL‐12 on IL‐10 Production and Proliferation of Human Tumour Reactive T Cells
Author(s) -
HEIKE M.,
SCHLAAK J.,
HEYL S.,
SCHULZEBERGKAMEN H.,
SCHMITT U.,
MEYER ZUM BU¨SCHENFELDE K.H.
Publication year - 1997
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1997.d01-386.x
Subject(s) - cytokine , biology , cell growth , t cell , interleukin 4 , immunology , microbiology and biotechnology , cancer research , immune system , biochemistry
The cytokine profile of tumour reactive T cells is likely to play a central role in their function. However, little is known about how cytokine patterns of tumour reactive T cells can be regulated. Here, the authors investigated the influence of exogenous regulatory cytokines in addition to interleukin‐2 (IL‐2) on cytokine patterns and the proliferation of T cells recognizing an autologous sarcoma cell line. In this system, IL‐4 and IL‐12 showed the most polarizing influences on tumour reactive T cells. Exogenous IL‐4 induced a predominant production of IL‐4 while decreasing the interferon‐γ (IFN‐γ) and IL‐10 production by tumour reactive T cells. It also stimulated the growth of tumour reactive CD4 + T cell clones. In contrast, IL‐12 substantially increased the production of IL‐10 and IFN‐γ. This was accompanied by a growth inhibition of tumour reactive T cells. The growth of CD4 + tumour reactive T cells was also suppressed by exogenous IL‐10. This study shows that cytokine patterns and proliferation tumour reactive T cells can be significantly influenced by exogenous cytokines and confirms the hypothesis of a negative feedback loop of IL‐12 by the induction of IL‐10 in the context of human tumour reactive T cells.