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HIV Antigens and T‐Cell Receptor Variable Beta Chain Families
Author(s) -
JASON J.,
INGE K. L.,
ORLOFF S. L.
Publication year - 1997
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1997.d01-368.x
Subject(s) - t cell receptor , superantigen , antigen , cd8 , beta (programming language) , biology , hiv antigens , monoclonal antibody , virology , antibody , virus , immunology , t cell , microbiology and biotechnology , immune system , viral disease , computer science , programming language
The authors investigated whether the human immunodeficiency virus (HIV) has restrictive effects on the variable region of the β chain (Vβ) of the T‐cell antigen receptor (TCR), by in vitro cultivation of non‐HIV‐infected peripheral blood lymphocytes with one of six HIV antigens or heat‐inactivated whole virus (HIV‐HI). Resting and blastic CD4 + and CD8 + cells were assessed with 3‐colour cytofluorometry and monoclonal antibodies to various Vβ families/subfamilies. The Vβ families affected include V β 's 13.1/.3, 8, and 21 with gp120; V β 21 with gp160 and RT; V β 8 with p25; V β 's 8 and 21 with Rev; and V β 's 3 and 21 with HIV‐2 Vpx. Vβ family‐specific effects with HIV‐HI did not differ significantly from those found with IL‐2 stimulation. Findings differed between CD4 + and CD8 + cells. For CD4 + lymphocytes, significant Vβ‐specific decreases were found, not the expansions found with superantigens or mitogens. CD8 + lymphocytes showed slight but significant expansions. The effects on V β 's 8, 13, and 21 are consistent with previous studies of HIV‐infected persons. However, it is difficult to accept that antigens encoded by different HIV genetic regions cause proportionate diminutions of similar Vβ families. The authors suggest that these effects may be secondary to changes in cytokine profiles rather than direct interactions with TCR Vβ's.