Premium
Identification and Characterization of Specific Receptor for Interleukin‐8 from the Surface of Human Monocytes
Author(s) -
BISHAYI B.,
SAMANTA A. K.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-69.x
Subject(s) - receptor , monocyte , cell surface receptor , cytokine , microbiology and biotechnology , intracellular , interleukin , interleukin 6 receptor , common gamma chain , biology , chemistry , interleukin 21 receptor , biophysics , immunology , biochemistry
A specific receptor for interleukin‐8 has been identified on the surface of human monocytes using 125 I IL‐8 as a probe. A binding kinetic pattern shows that saturation was attained after 90 min and that the receptor was distinct from the receptors of other cytokines (IL‐1α, IL‐2, TNFα, GMCSF) and FMLP. Scatchard analysis of the binding data shows that 7000–10,000 receptors/monocyte are present with an equilibrium Kd 7 × 10 −9 M . By immunoblot, the receptor for IL‐8 showed a sharp band with approximate M.W. 59 kD, consistent with the M.W. of IL‐8 receptor of neutrophils. In Boyden Chamber, monocytes migrated towards IL‐8 and the cytokine was observed to induce transient rise of intracellular Ca ++ in the cells. Thus, identification of functionally active IL‐8 receptor in monocyte may be helpful for understanding its possible role during inflammation.