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Adjuvant Properties of Montanide CSA 720 with a Recombinant HIV P17 gag Protein and Synthetic Peptide Antigens
Author(s) -
KIRKLEY J. E.,
GOLDSTEIN A. L.,
NAYLOR P. H.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-60.x
Subject(s) - adjuvant , immunogen , antibody , antigen , alum , recombinant dna , virology , peptide , immunization , antibody titer , titer , medicine , immunology , chemistry , monoclonal antibody , biochemistry , organic chemistry , gene
The adjuvant properties of Montanide CSA 720 were assessed in a comparison with alum. BALB/c mice were immunized with recombinant HIV‐1 gag protein p17 administered in either of the two adjuvants. The serum antibody response to p17 with Montanide CSA 720 appeared faster and reached a higher titre than with alum. The serum antibody response to p17 in Montanide CSA 720 was further characterized by a higher titre antibody directed against a 30 amino acid segment from the entire protein. The Montanide CSA 720 adjuvant was sufficiently strong to induce an antibody response against a weak synthetic peptide immunogen after two immunizations, while immunization with the peptide in alum generated no detectable serum antibody. The p17‐specific proliferative response of splenocytes from animals immunized with recombinant protein in either adjuvant was similar.