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GENOMIC ORGANIzATION AND PROMOTER ANALYSIS OF THE GENE ifngr2 ENCODING THE SECOND CHAIN OF THE MOUSE INTERFERON‐γ RECEPTOR
Author(s) -
Ebensperger C.,
RHEE S.,
Muthukumaran G.,
Lembo D.,
Donnelly R.,
Pestka S.,
Dembic Z.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-353.x
Subject(s) - biology , microbiology and biotechnology , gene , promoter , chinese hamster ovary cell , tata box , cosmid , exon , transcription (linguistics) , genomic dna , response element , reporter gene , caat box , luciferase , genomic library , genetics , transfection , gene expression , receptor , peptide sequence , linguistics , philosophy
A clone containing the gene ifngr2 for the second chain (IFN‐γR2) of the mouse interferon γ receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5′‐flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP‐2, NF1, EGR and NFκB. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells.