Premium
Expression and Regulation of Constitutive and Acute Phase Serum Amyloid A mRNAs in Hepatic and Non‐Hepatic Cell Lines
Author(s) -
STEEL D. M.,
DONOGHUE F. C.,
O'NEILL R. M.,
UHLAR C. M.,
WHITEHEAD A. S.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-341.x
Subject(s) - cell culture , serum amyloid a , messenger rna , hepatic stellate cell , cell , endocrinology , polyadenylation , microbiology and biotechnology , medicine , biology , chemistry , immunology , gene , inflammation , biochemistry , genetics
‘Acute phase’ and ‘constitutive’ SAA (A‐SAA and C‐SAA, respectively) mRNA levels were measured in hepatic and non‐hepatic cell lines after treatment with monocyte conditioned medium (MoCM), with or without dexamethasone (Dex). A‐SAA mRNAs were detected in MoCM‐treated hepatoma cell lines (PLC/PRF/5, HuH7, HepG2, and Hep3B), a fibroblast cell line (MRC5), six epithelial cell lines (RT4/31, SW13, Hela Ohio, HCT‐8, CaCo2, and KB), and an endothelial cell line ECV304. In KB cells, Dex alone caused a dramatic increase in A‐SAA mRNA levels. C‐SAA was detected in all hepatic and non‐hepatic cell lines. Two differentially regulated size classes of C‐SAA mRNA were detected in the hepatoma cell lines. A‐SAA mRNA levels were measured in ECV304 cells treated with IL‐1β, IL‐6, TNFα and Dex, in various combinations, and revealed different profiles to those seen for hepatic cells. The extent of polyadenylation of A‐SAA mRNA in ECV304 and KB cells differed whereas the polyadenylation of C‐SAA mRNA remained constant. These data suggest that the parameters that determine the steady state mRNA levels and post‐transcriptional regulation of A‐SAA and C‐SAA mRNAs are different and are cell type specific.