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Activity Inhibition of Cytolytic Lymphocytes by Omeprazole
Author(s) -
SCARINGI L.,
CORNACCHIONE P.,
FETTUCCIARI K.,
ROSATI E.,
ROSSI R.,
MARCONI P.,
CAPODICASA E.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-300.x
Subject(s) - cytolysis , omeprazole , immunology , chemistry , biology , pharmacology , cytotoxicity , biochemistry , in vitro
This study examined the in vitro effect of omeprazole (OM) on various types of murine cytocidal lymphocytes. The results show that OM caused a strong inhibition of basal natural killer (NK) activity in spleen cells (SC) from untreated CD2F1 mice; in peritoneal exudate cells and SC activated in vivo by injection of maleic anhydride divinyl ether 1,2‐copolymer (MVE‐2) or inactivated Candida albicans (CA); in lymphokine‐activated killer (LAK) activity generated in vitro from splenocytes cultured with rhIL‐2 and in allo‐specific cytotoxic lymphocyte‐mediated lysis generated in vitro . A significant inhibition of cytotoxic activity of all types of effector cells after 30 min incubation was already induced by OM at 1 × 10 −3 m concentration, after 1 h incubation at 5 × 10 −4 m and after 4 h incubation at 1 × 10 −4 m OM. Complete inhibition of lytic activity was obtained after 4 h incubation of effector cells with 1 × 10 −3 m OM. No inhibitory effect was observed at 5 × 10 −5 m OM concentration. Indomethacin did not abrogate the OM inhibitory effect on NK/LAK activity, suggesting that prostaglandins are not involved in the process leading to suppression of cytocidal activity. When effector cells were incubated with OM in presence of rhIL‐2 (500 U/ml), the cytokine failed to antagonize the inhibitory effect of the drug. On the contrary, if OM pretreated cells were incubated with rhIL‐2 for a further 18 h after drug removal, this cytokine was able to restore NK activity, but only when NK inhibition was incomplete. These results demonstrate for the first time that in vitro OM causes a rapid, strong effect on various types of cytotoxic lymphocytes ranging from cytotoxicity inhibition to irreversible cell damage.