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Strict Adherence to a Common Rank Order of T‐Cell Receptor Vβ Usage in Human Leucocyte Antigen Disparate Individuals
Author(s) -
BRINCHMANN J. E.,
JANSON C. H.,
KLEM L. B.,
HAAHEIM L. L.,
SPURKLAND A.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-294.x
Subject(s) - cd8 , t cell receptor , cd28 , antigen , biology , cytotoxic t cell , human leukocyte antigen , immunology , t cell , beta (programming language) , monoclonal antibody , antibody , microbiology and biotechnology , genetics , immune system , in vitro , computer science , programming language
In order to investigate the T‐cell receptor (TCR) Vβ usage in different T‐cell subsets, the authors performed flow cytometric analyses using a large panel of TCR Vβ‐specific monoclonal antibodies on CD4 + , CD8 + CD28 + and CD8 + CD28 − T cells from 15 random blood donors, six umbilical cords and seven human leucocyte antigen (HLA) identical non‐twin sibling pairs. The authors found that the proportion of T cells expressing each Vβ gene product was similar within CD4 + and CD8 + CD28 + T cells from all samples studied. For these T‐cell subsets a rank order of Vβ usage could be identified which was adhered to by all donors. In contrast, within CD8 + CD28 − T cells a wide variation of Vβ usage was found between individuals, and no rank order correlation could be detected. Members of HLA identical sibling pairs were found to be no more similar in their usage of Vβ gene products than pairs of HLA disparate random blood donors. Groups of individuals sharing HLA antigens were no different from the groups not sharing such antigens in their usage of Vβ segments. The results suggest that HLA polymorphisms play no more than a minor part in determining TCR Vβ usage.