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Activation of Cloned Human CD4 + Th 1 and Th 2 Cells by Blood Dendritic Cells
Author(s) -
ROTH R.,
SPIEGELBERG H. L.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-270.x
Subject(s) - microbiology and biotechnology , chemistry , biology , radiochemistry
Dendritic cells (DC) have been reported to be the most potent antigen‐presenting cells (APC) for the activation of naive T cells and to be 10–100‐fold more potent APC than monocytes (Mφ) in the mixed lymphocyte reaction. In this study the authors compared human blood DC with Mφ and B cells for their ability to activate cloned rye grass allergen Lol p I specific CD4 + Th 1 and Th 2 cells. In the presence of Lol p I, all three types of APC activated Th 1 and Th 2 cells to a similar extent, as shown by T‐cell proliferation and interferon‐γ, interleukin‐2 (IL‐2) or IL‐4 secretion. However, at low APC : T cell ratios, Mφ were the most potent APC for both Th 1 and Th 2 cells followed in decreasing order by DC and B cells. This hierarchy was observed with APC preparations isolated by negative selection or highly purified by positive selection using fluorescent cell sorting for HLA‐DR high ‐DC, CD14 pos ‐Mφ and CD19 pos ‐B cells. The data demonstrate that, in contrast to what has been reported for naive T cells, human blood DC activate cloned memory Th 1 and Th 2 cells to a similar extent as Mφ and B cells presumably because the requirements for activation of memory type T cells are less stringent than those for naive T cells.