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Heterogeneous Expression of Recombination Activating Genes and Surface CD5 in CD3 low CD4 + CD8 + Thymocytes
Author(s) -
SHEARD M.,
SUGAYA K.,
FURUTA M.,
TSUDA S.,
TAKAHAMA Y.
Publication year - 1996
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1996.d01-263.x
Subject(s) - cd5 , cd8 , recombination , microbiology and biotechnology , cd3 , gene , biology , cytotoxic t cell , gene expression , thymocyte , physics , genetics , flow cytometry , immune system , in vitro
Clonal selection in the thymus occurs mostly in the CD4 + CD8 + (double positive; DP) stage. Within DP thymocytes, cells in the CD3 low subset are believed to be involved in clonal selection, and this subset is generally considered as a homogeneous population. T‐cell antigen‐receptor (TCR) signals on DP thymocytes are known to (i) down‐regulate recombination activating gene (RAG) expression; and (ii) up‐regulate the expression of T‐cell function‐associated molecules, including the cell surface glycoprotein CD5. The present study examined the expression of RAG and CD5 molecules among the subpopulations of normal adult DP thymocytes. DP thymocytes were fractionated according to their cell surface expression levels of TCR‐CD3 complex into CD3 dim , CD3 lo , CD3 med , and CD3 hi cells, since TCR expression is known to increase during thymocyte maturation. Down‐regulation of RAG mRNA was located between the CD3 low and CD3 med DP populations. However, within the DP CD3 low subpopulation, we found that CD5 varies from low to high expression levels. Upon fractionation of DP CD3 low thymocytes into CD5 low and CD5 high subsets, we were able to detect down‐regulation of RAG transcripts within the CD3 low subpopulation. Thus, by the criteria of CD5 surface expression and RAG mRNA expression levels, DP CD3 low thymocytes can be considered a heterogeneous thymocyte subpopulation.