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Double‐stranded conformation polymorphism of heterologous RNA transcripts and its use for virus strain differentiation
Author(s) -
Arie Rosner,
Ludmila Maslenin,
S. Spiegel
Publication year - 1999
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1046/j.1365-3059.1999.00335.x
Subject(s) - heterologous , biology , rna , genetics , microbiology and biotechnology , messenger rna , virus , gene
Polymorphism of heterologous duplexes of RNA transcripts of PNRSV isolates was demonstrated. Complementary RNA trancripts were copied from PCR products of PNRSV isolates, to which the T7 RNA promoter was added on either the 3′ or 5′‐ends. Heterologous duplexes made from complementary RNA transcripts originating from several virus isolates differed in their electrophoretic mobilities. This polymorphism is hypothesized to be conferred by conformational variations among the transcript duplexes, and was termed by us ‘double‐stranded transcript conformation polymorphism (ds‐TCP)’. This method may serve as an additional tool in virus strain differentiation.