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Characterization of cucumber mosaic cucumovirus isolates in Greece
Author(s) -
Varveri,
Boutsika
Publication year - 1999
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1046/j.1365-3059.1999.00308.x
Subject(s) - cucumovirus , biology , cucumber mosaic virus , restriction enzyme , virology , restriction fragment length polymorphism , serotype , outbreak , virus , polymerase chain reaction , gene , plant virus , genetics
Since 1990, massive cucumber mosaic cucumovirus (CMV) outbreaks of various symptomatology, especially on tomato, have been observed all over Greece. To characterize local virus populations, 40 CMV isolates of different origin and symptomatology in the field and glasshouse were examined for subgroup identification and presence of satellite RNA (satRNA). The IC‐PCR method was used to amplify part of the coat protein gene of the isolates; the products were submitted to RFLP analysis using Eco RI and Msp I restriction enzymes. Most isolates gave the characteristic pattern attributed to CMV subgroup I, confirming the results obtained by serotyping with monoclonal antibodies. Some Greek isolates, however, possessed additional Eco RI and Msp I sites unusual for subgroup I isolates, and the patterns obtained, especially for Msp I, could be confused with those of subgroup II. These data suggest that restriction enzyme analysis of amplified PCR products used for strain characterization needs to be treated with caution, especially for viruses such as CMV showing genomic heterogeneity. IC‐PCR was also developed for satRNA amplification and the results agreed with those of molecular hybridization with a dig‐DNA probe. A satRNA was carried by 77% of the isolates.