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Phaseollidin stored in vacuoles and the phytoalexin response in bean
Author(s) -
SorianoRichards E.,
UribeSalas D.,
IbarraBarrera G.
Publication year - 1998
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1046/j.1365-3059.1998.00264.x
Subject(s) - phytoalexin , biology , colletotrichum lindemuthianum , vacuole , hypocotyl , elicitor , botany , biochemistry , cultivar , cytoplasm , gene , resveratrol
In time‐course experiments, the amounts of phytoalexin in hypocotyls of the resistant bean cultivar Flor de Mayo were determined after infection with Colletotrichum lindemuthianum . Phaseollin and phaseollidin accumulated as a defence response. However, phaseollidin accumulated earlier than phaseollin and at greater concentrations (155 + 14 compared with 120 + 9 μ g g −1 fresh weight). Phaseollidin was found conjugated as a glucoside in the tissue. Fungal treatment apparently led to a decline of approximately 50% in phaseollidin conjugate after only 7 h after infection. Isolation of vacuoles confirmed the presence of phaseollidin glucosides in this organelle. Treatment of the tissue or isolated protoplasts with a fungal elicitor also produced a decrease, by half, of phaseollidin conjugate concentrations from vacuoles isolated from both sources. The contribution of pre‐existing pools of phaseollidin glycoside to the accumulation of this phytoalexin is discussed.