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Genetic comparison of the peach yellow leaf roll agent with European fruit tree phytoplasmas of the apple proliferation group
Author(s) -
KISON H.,
KIRKPATRICK B. C.,
SEEMÜLLER E.
Publication year - 1997
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1046/j.1365-3059.1997.d01-35.x
Subject(s) - biology , restriction fragment length polymorphism , phytoplasma , southern blot , phylogenetic tree , pear , ribosomal dna , ribosomal rna , dna , restriction enzyme , genetics , botany , microbiology and biotechnology , polymerase chain reaction , gene
Restriction fragment length polymorphism (RFLP) analysis of PCR‐amplified ribosomal DNA and Southern blot hybridization using cloned chromosomal DNA fragments from the apple proliferation (AP) phytoplasma as probes were used to investigate the genetic relationship of the California peach yellow leaf roll (PYLR) agent with phytoplasmas causing fruit tree diseases in Europe. This comparison showed that the California PYLR phytoplasma is closely related to apple proliferation (AP), pear decline, and European stone fruit yellows phytoplasmas and that it is a member of the phylogenetic AP group. The PYLR agent could clearly be distinguished from the AP and European stone fruit yellows phytoplasmas by Southern blot hybridization with DNA fragments from the AP phytoplasma and by RFLP analysis of ribosomal DNA employing Ssp I, Bsa AI, and Rsa I restriction endonucleases. However, the PYLR phytoplasma was indistinguishable from the pear decline agent by RFLP analysis of PCR‐amplified ribosomal DNA.