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Elimination of phytoplasma by stem culture from mulberry plants ( Morus alba ) with dwarf disease
Author(s) -
DAI Q.,
HE F.T.,
LIU P.Y.
Publication year - 1997
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1046/j.1365-3059.1997.d01-202.x
Subject(s) - phytoplasma , biology , botany , polymerase chain reaction , dapi , tissue culture , in vitro , staining , genetics , gene , restriction fragment length polymorphism
Stem culture in vitro was attempted for the elimination of mulberry dwarf phytoplasma. Stem segments, from mulberry plants ( Morus alba ) seriously infected with the phytoplasma, were cultured on Murashige and Skoog (MS) solid nutrient medium without phytohormones for 2–3 months. The tissue cultures, and regenerated plants grown under greenhouse conditions for 3 years, were tested by polymerase chain reaction (PCR) and 4,6‐diamidino‐2‐phenylindole (DAPI) staining for the presence of the phytoplasma, and also observed for mulberry dwarf symptoms. From 70% to 90% of regenerated plants were apparently freed from the phytoplasma since they remained PCR‐negative, DAPI‐negative and symptom‐free for as long as tested (3 years).