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Vegetative incompatibility in Diaporthe ambigua
Author(s) -
SMIT W. A.,
WINGFIELD B. D.,
WINGFIELD M. J.
Publication year - 1997
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1046/j.1365-3059.1997.d01-19.x
Subject(s) - biology , homothallism , ascospore , rootstock , pear , botany , horticulture , mating type , spore , biochemistry , gene
Vegetative compatibility of strains of Diaporthe ambigua has not previously been examined. Single ascospore and single ascus strains, originating from individual apple, pear and plum rootstocks, were paired on freshly prepared oatmeal agar to determine if vegetative incompatibility could be detected in D. ambigua . Barrage reactions were evident as black lines along the zone of mycelial contact between expanding colonies (vegetative incompatibility reaction). Strains from cankers within an area were of numerous vegetative compatibility groups (VCGs). Strains from adjacent rootstocks usually differed in VCG. D. ambigua has the ability to outcross, and does so, despite its apparent homothallic nature. DsRNA‐containing strains of D. ambigua developed a broad, clear zone when paired with a dsRNA‐containing strain from a different VCG.