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Electrophysiological localization of blue light sensory sites in etiolated dicotyledon seedlings
Author(s) -
BABOURINA O. K.,
NEWMAN I. A.,
SHABALA S. N.
Publication year - 2003
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.2003.01072.x
Subject(s) - epicotyl , etiolation , hypocotyl , cucumis , pisum , phaseolus , botany , biology , seedling , biophysics , sativum , biochemistry , enzyme
Changes induced by blue light (BL) in ion fluxes and pH in etiolated seedlings of dicotyledons ( Cucumis sativus L., Phaseolus lunatus L., Pisum sativum L.) were studied using a non‐invasive ion‐selective microelectrode technique (MIFE). BL induced significant Ca 2+ uptake within the first 5 min after onset in upper parts of the whole cucumber seedling. To locate the sites of the BL perception, studies on excised segments were performed. The data obtained demonstrated that the observed Ca 2+ uptake is associated with the very top of the hypocotyl hook of cucumber and butter bean, and with the whole epicotyl of pea, although the response was stronger for the upper parts of the epicotyl. Addition of Gd 3+ and La 3+ into the bathing solution completely prevented Ca 2+ influx, implicating the involvement of Ca 2+ channels in the regulation of Ca 2+ transport during BL exposure. BL‐induced changes in H+ and Ca 2+ fluxes were not likely to be linked to the one mechanism, as they were separated spatially and pharmacologically.