Premium
Transcription factor genes with expression correlated to nitrate‐related root plasticity of Arabidopsis thaliana
Author(s) -
TRANBARGER T. J.,
ALGHAZI Y.,
MULLER B.,
TEYSSENDIER DE LA SERVE B.,
DOUMAS P.,
TOURAINE B.
Publication year - 2003
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.2003.00977.x
Subject(s) - primordium , arabidopsis thaliana , lateral root , biology , botany , transcription factor , arabidopsis , glutamine , gene expression , gene , phloem , microbiology and biotechnology , biochemistry , amino acid , mutant
The root response to changes in nitrogen availability was examined in Arabidopsis thaliana . Plants grown on vertically oriented Petri plates with nutrient media containing either 10 or 0.5 m m KNO 3 supplemented with 5 m m glutamine were analysed for changes in root architecture, morphology and nitrogen status compared to plants grown on 0.5 m m KNO 3 . Fourteen‐day‐old 10 m m KNO 3 ‐grown plants displayed a decrease in number, length and density of lateral roots, had the highest tissue NO 3 – content, but no change in amino acid composition. Lateral root development was arrested at a stage after the initiation of primordia but prior to emergence. In contrast, plants grown on 0.5 m m KNO 3 supplemented with glutamine had a slightly higher number and density of lateral root primordia and lateral roots, lower root NO 3 – content, and higher tissue glutamine content. A macro‐array containing 126 putative A. thaliana transcriptional factor expressed sequence tags was used to screen for regulatory genes with expression correlated to the root plasticity response. Genes for a basic leucine zipper (bZIP) and a LIM transcription factor were identified and via Northern analysis it was confirmed that their expression is preferentially observed in the roots and correlated to the root response to nitrate availability.