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Nuclear magnetic resonanceimaging of membrane permeability changes in plants during osmoticstress
Author(s) -
Van Der Weerd L.,
Claessens M. M. A. E.,
Efdé C.,
Van As H.
Publication year - 2002
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.2002.00934.x
Subject(s) - pennisetum , permeability (electromagnetism) , water transport , water content , membrane , chemistry , water stress , sugar , relaxation (psychology) , water balance , stress relaxation , nuclear magnetic resonance , horticulture , biophysics , botany , materials science , biology , water flow , food science , soil science , physics , biochemistry , environmental science , geotechnical engineering , neuroscience , engineering , creep , composite material
The cell water balance of maize ( Zea mays L., cv LG 11) andpearl millet ( Pennisetum americanum L., cv MH 179) duringosmotic stress was studied non‐invasively using 1 H nuclearmagnetic resonance (NMR) microscopy. Single NMR parameter imagesof (i) the water content (ii) the transverse relaxation time ( T 2 )and (iii) the apparent diffusion coefficient ( D app )were used to follow the water status of the stem apical region duringosmotic stress. During stress there are hardly any changes in watercontent or T 2 of the stem region of maize. Incontrast, the apical tissue of pearl millet showed a ∼ 30% decreaseof T 2 within 48 h of stress, whereasthe water content and D app did not change. Thesechanges can be explained by an increase of the membrane permeabilityfor water. This conclusion is supported by results from scanningelectron microscopy, relaxation measurements of sugar solutionsand numerical simulations of the relaxation and (apparent) diffusionbehaviour of water in a plant cell.