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Suramin inhibits oxidant signalling in tobacco suspension‐cultured cells
Author(s) -
Miles G. P.,
Samuel M. A.,
Ellis B. E.
Publication year - 2002
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.2002.00841.x
Subject(s) - suramin , mapk/erk pathway , microbiology and biotechnology , reactive oxygen species , chemistry , signal transduction , hydrogen peroxide , receptor , oxidative stress , biochemistry , biology
Abstract Plant cells respond to ultraviolet radiation and other oxidant‐generating agents by mobilizing cellular defences, but the signal network linking perception of redox perturbation with defence remains unknown. Irradiation of tobacco suspension‐cultured cells with UVC was found to induce the activation of a specific MAPK 46 (salicylic acid‐induced protein kinase) within 1 min. To explore where UVC and other oxidants might initially act to trigger this signal response, we employed suramin, a non‐membrane‐permeable reagent that interferes with membrane receptor‐mediated signalling in mammalian cells. Pre‐treatment of tobacco cells with suramin strongly attenuated the UVC‐induced activation of MAPK 46 in a concentration‐dependent manner. Suramin was also able to interdict both ozone‐ and hydrogen peroxide‐induced activation of MAPK 46 , indicating that reactive oxygen species (ROS) signalling to the MAPK cascade in general may be initiated at the cell membrane, perhaps through oxidative activation of membrane receptors.