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Sexual reproduction of Lolium perenne L. and Trifolium repens L. under free air CO 2 enrichment (FACE) at two levels of nitrogen application
Author(s) -
Wagner J.,
Lüscher A.,
Hillebrand C.,
Kobald B.,
Spitaler N.,
Larcher W.
Publication year - 2001
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.2001.00740.x
Subject(s) - lolium perenne , trifolium repens , biology , anthesis , agronomy , repens , inflorescence , legume , perennial plant , germination , sexual reproduction , reproduction , botany , cultivar , ecology
Field experiments in managed grassland have shown that the response of vegetative growth to elevated CO 2 is nitrogen‐dependent in grasses, but independent in N 2 ‐fixing legumes. In the present study, we tested whether this is also true for reproduction. We evaluated reproductive growth, flowering phenology, seed development, reproductive success and seed germination in the grass Lolium perenne L. and the legume Trifolium repens L., growing in monocultures in a free air carbon dioxide enrichment (FACE) system at ambient (35 Pa) and elevated (60 Pa) partial pressure of CO 2 and two levels of nitrogen fertilization (14 and 56 g N m −2 a −1 ). In both species, elevated CO 2 had no significant effect on sexual reproduction. In L. perenne , reproduction was mainly nitrogen‐dependent. The weak interactions between CO 2 and mineral N supply (13% more flowers and 8% more grains per spike at high N, 7% less flowers and 8% less grains at low N) were not significant. Under elevated CO 2 , grain maturation was slightly enhanced and grain weight tended to decrease. No influence could be ascertained in the date of anthesis, the temporal pattern of grain growth, the rate of grain abortion and germination. Trifolium repens , grown under CO 2 enrichment at both levels of N fertilization, flowered 10 d earlier, tended to form more inflorescences per ground area and more flowers (8–12%) and seeds (>18%) per inflorescence than at ambient CO 2 . The temporal pattern of seed growth was about the same in all treatments; embryo development, however, was accelerated in fumigated plants. The number of aborted seeds per pod, seed size, thousand‐seed weight and germinability did not show any influence of CO 2 . Fumigated plants at high N were attacked slightly more frequently by seed‐eating weevils, which lowered the seed output per pod. In summary, the reproductive response of L. perenne and T. repens to CO 2 enrichment on the flower and inflorescence level was far weaker than expected from the results on vegetative growth.