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Resistance of cold‐hardened winter rye leaves ( Secale cereale L.) to photo‐oxidative stress
Author(s) -
Streb P.,
Shang W,
Feierabend J
Publication year - 1999
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.1999.00483.x
Subject(s) - secale , catalase , glutathione reductase , paraquat , superoxide dismutase , xanthophyll , chlorophyll , chloroplast , photoprotection , zeaxanthin , oxidative stress , antioxidant , carotenoid , photosystem ii , chemistry , biochemistry , photoinhibition , biology , photosynthesis , botany , lutein , glutathione peroxidase , gene
Catalase and photosystem II (PSII) were strongly inactivated during exposure to 4 °C and moderate light in 22 °C‐grown non‐hardened leaves (NHL) of winter rye ( Secale cereale L.), but highly resistant to photo‐inactivation at low temperature in 4 °C‐grown cold‐hardened leaves (CHL). Resistance of CHL to chilling‐induced photo‐inactivation of catalase and PSII depended partially on more efficient de novo synthesis at 4 °C and partially on improved protection. Lower rates of chloroplast‐mediated inactivation of catalase in vitro indicated that less reactive oxygen was released by chloroplasts from CHL than by chloroplasts from NHL. The contents of xanthophyll cycle carotenoids, α ‐tocopherol, ascorbate, glutathione, the activities of superoxide dismutase and glutathione reductase, and the tolerance against paraquat‐induced photo‐oxidative damage were greatly increased in CHL, relative to NHL. Zeaxanthin‐related thermal energy dissipation was only of minor importance for paraquat‐tolerance and protection of catalase in CHL. When CHL were transferred to a higher temperature of 22 °C the increased resistance to photo‐inactivation of catalase and PSII and the increased paraquat‐tolerance were largely lost within 3 d, whereas most non‐enzymic and enzymic antioxidants retained higher levels than in NHL. The decline of resistance to photodamage during dehardening was not related to concomitant changes of antioxidants or antioxidative enzymes.