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An improvement of the method for calibrating measurements of photosynthetic CO 2 flux
Author(s) -
Hari P.,
Keronen P.,
Bäck J.,
Altimir N.,
Linkosalo T.,
Pohja T.,
Kulmala M.,
Vesala T.
Publication year - 1999
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.1999.00478.x
Subject(s) - cuvette , calibration , repeatability , analyser , flux (metallurgy) , analytical chemistry (journal) , accuracy and precision , biological system , materials science , chemistry , process engineering , environmental science , optics , physics , chromatography , engineering , quantum mechanics , metallurgy , biology
Measurements of rapid changes in concentrations and fluxes of gaseous compounds relating to photosynthetic gas exchange are commonly performed using flow‐through cuvettes in connection with infrared gas analysers. The accuracy and repeatability of these measurements relies ultimately upon the design of the system as a whole, rather than upon each of its components, and therefore the calibration and testing of the system should be performed keeping this in mind. We present here a simple and efficient method for the calibration of such a measurement system using a precisely determined CO 2 flow. This method gives us the opportunity to take into account any disturbing effects caused by undesired properties of the chamber or tubing materials. With the proposed calibration method, the accuracy of the CO 2 flux measurement is improved from 8% up to the level of 2%, determined mainly by the accuracy of the control gas used for calibration of the CO 2 analyser.