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Hydraulic contribution in cell elongation of tissue‐cultured plants: growth retardation induced by osmotic and temperature stresses and addition of 2,4‐dichlorophenoxyacetic acid and benzylaminopurine
Author(s) -
Ikeda T.,
ami H.,
Fukuyama T.,
Hashimoto Y.
Publication year - 1999
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.1999.00463.x
Subject(s) - phaseolus , elongation , tissue culture , nutrient , growth rate , stress (linguistics) , horticulture , biophysics , osmotic pressure , osmotic shock , germination , water stress , biology , botany , biochemistry , materials science , in vitro , ecology , composite material , linguistics , philosophy , geometry , mathematics , gene , ultimate tensile strength
This work was undertaken to determine the growth parameters of Lockhart’s equation for finding which component was predominantly contributing to the cell expansion rates of plants subjected to environmental stresses under tissue‐culture conditions. Embryos isolated from soybean ( Glycine max [L.] Merr.) and kidney bean ( Phaseolus vulgaris L.) seeds were grown under tissue‐culture conditions. The water potential of culture media ranged from − 0·02 to − 0·94 MPa so that nutrient deficiency and salt stress conditions could be applied. Additionally, the temperature of culture conditions was set from 10 to 40 °C to apply low‐temperature and high‐temperature stresses on plants grown at the optimum concentration of culture medium. Cell expansion could be inhibited completely by adding 2,4‐dichlorophenoxyacetic acid and benzylaminopurine to culture media to form callus tissue. The sizes of the water potential gradient between the water source and elongating cells correlated with the speed of growth rates under nutrient deficiency, salt stress, growth retardation induced by plant hormones, low‐temperature and high‐temperature conditions in the present study, indicating that cell expansion rates were mainly associated with how much water could be absorbed by elongating cells regardless of the kinds of environmental stress conditions applied.