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Effects of ozone on the plasma membrane proteins in Arabidopsis thaliana (L.) leaves
Author(s) -
TOKARSKASCHLATTNER M.,
FINK A.,
CASTILLO F. J.,
CRESPI P.,
CREVECOEUR M.,
GREPPIN H.,
TACCHINI P.
Publication year - 1997
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1046/j.1365-3040.1997.d01-149.x
Subject(s) - arabidopsis thaliana , membrane , fumigation , ozone , chemistry , arabidopsis , polyacrylamide gel electrophoresis , biochemistry , membrane protein , botany , chromatography , biology , gene , horticulture , organic chemistry , mutant , enzyme
The protein pattern of leaf plasma membranes from Arabidopsis thaliana (L.) Landsberg erecta was analysed in order to detect changes induced by acute short‐term ozone treatment. Plasma membranes were isolated 0, 3 and 8 h after the end of a 2 h fumigation of the plants with 500 nmol mol −1 of O 3 . Proteins extracted from plasma membranes were separated by high‐performance two‐dimensional polyacrylamide gel electrophoresis. Eight hours after the end of fumigation, one new protein appeared and the amounts of two other proteins increased significantly. The reported study is a first step towards the identification of plasmalemma proteins altered by ozone and to a more detailed characterization of structural changes occurring in the plasma membrane after ozone exposure.