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Visualizing the postembryonic development of Sarcophaga peregrina (flesh fly) by NMR microscopy
Author(s) -
Price William S.,
Kobayashi Ayako,
Ide Hiroyuki,
Natori Shunji,
Arata YojI.
Publication year - 1999
Publication title -
physiological entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 57
eISSN - 1365-3032
pISSN - 0307-6962
DOI - 10.1046/j.1365-3032.1999.00156.x
Subject(s) - biology , flesh fly , pupa , larva , epidermis (zoology) , anatomy , flesh , imaginal disc , metamorphosis , drosophila melanogaster , botany , biochemistry , food science , gene
The postembryonic development of the flesh fly was studied using high resolution nuclear magnetic resonance imaging. Because this development occurs in a puparium, this process cannot be observed directly using standard histological techniques. The remodelling of histolysing larval tissues to developing imaginal tissues including the yellow body, a transient alimentary structure, and the integration of the developed adult structure were revealed in the images. Most surprisingly it was found that a large gas space that forms in the central region of the prepupa moves to the dorso‐anterial region in less than 5 min due to the larval–pupal apolysis together with separation of the developing pupal epidermis from the puparium.