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Neutropenia augments experimentally induced Schistosoma japonicum egg granuloma formation in CBA mice, but not in C57BL/6 mice
Author(s) -
Hirata Mizuki,
Hara Tatsuru,
Kage Masayoshi,
Fukuma Toshihide,
Sendo Fujiro
Publication year - 2002
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.2002.00491.x
Subject(s) - schistosoma japonicum , biology , granuloma , c57bl/6 , immunology , granuloma formation , neutropenia , schistosomiasis , helminths , genetics , chemotherapy
SUMMARY The present study was designed to investigate the role of neutrophils during the development of Schistosoma japonicum egg granulomas, in C57BL/6 and CBA mice. Laid eggs were implanted into the liver and monoclonal antibody, RB6‐8C5, was used to eliminate neutrophils. After daily antibody treatment between days 9 and 13 of egg implantation, both strains of mice showed a marked decrease in neutrophil infiltration and coagulative hepatocyte necrosis at 2 weeks. At 4 weeks, after antibody administration every other day between days 16 and 26, granuloma formation in C57BL/6 mice was not affected by the treatment, whereas CBA mice exhibited a significant increase of reactions. Neutropenia augmented the Th2 cytokine response (IL‐4, IL‐13 and IL‐5), but not for IFN‐γ at any time point examined and in either strain of mice. Higher levels of IL‐4 and IL‐13 were noted in CBA mice at early and late stages of granuloma formation, compared to C57BL/6 mice. There was also a striking difference in IL‐13 production between the two strains. Our results indicate that neutropenia is associated with a significant augmentation of S. japonicum egg‐induced granuloma formation in CBA mice, probably through increase in Th2 cytokines, however, the effects differ between early and late stages and between high and low responders.