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Gastro‐allergic anisakiasis as a consequence of simultaneous primary and secondary immune response
Author(s) -
Daschner Alvaro,
Cuéllar Carmen,
SánchezPastor Sofia,
Pascual CristinaYolanda,
MartínEsteban Manuel
Publication year - 2002
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.2002.00458.x
Subject(s) - immunoglobulin e , immunology , antibody , biology , allergy , immune system , antigen
Summary Gastro‐allergic anisakiasis has been reported as an entity in which an acute parasitism by Anisakis simplex is accompanied by an immunoglobulin (Ig)E‐mediated systemic allergic reaction. Serum samples were obtained from 24 patients within 24 h after the onset of symptoms (day 0) and after 1 month (day 30) and in 13 patients after 6 months. Total IgE was assessed by the Imx method. Specific IgE was assessed by CAP‐FEIA. Specific IgM, IgG, IgG4 and IgA antibodies were assessed by enzyme‐linked immunosorbent assay against crude extract (CE) and excretory–secretory products (ESP). IgE immunoblotting (IB) was directed against CE or ESP (day 0 and day 30). We found a rise of total IgE, specific IgE, number of bands in IgE‐IB, IgG and IgG4 between day 0 and day 30 with a fall to near basal levels after 6 months. IgM levels were highest at day 0, falling over the next 6 months and IgA levels remained almost unchanged. Correlation studies revealed a parallel stimulation of nearly all Ig isotypes, except IgM anti‐ESP, whose antibody levels correlated negatively with specific IgG levels. We found an extension of the IgE antibody repertoire in IB. We conclude that the allergic IgE‐mediated reaction in the course of gastro‐allergic anisakiasis involves a parallel secondary Th2 type memory response and a primary immunologic stimulation of both Th2 and Th1 lymphocyte subsets against previously unrecognized antigens.