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Cross‐protection studies with gut membrane glycoprotein antigens from Haemonchus contortus and Teladorsagia circumcincta
Author(s) -
Smith W.D.,
Pettit D.,
Smith S.K.
Publication year - 2001
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.2001.00375.x
Subject(s) - haemonchus contortus , teladorsagia circumcincta , biology , proteases , glycoprotein , antigen , microbiology and biotechnology , parasite hosting , helminths , immunology , enzyme , biochemistry , world wide web , computer science
Gel profiles of the peanut and ConA lectin binding integral membrane glycoproteins of Teladorsagia circumcincta and Haemonchus contortus were compared and found to be considerably different . However, some of the Teladorsagia polypeptides were recognized by antisera specific for Haemonchus amino‐, metallo‐ or aspartyl peptidases, enzymes which are known to be protective antigens for that parasite. As expected, an experimental vaccine containing these Haemonchus proteases was extremely effective against homologous challenge, reducing egg and worm counts by more than 99% and 92%, respectively, but it did not provide any useful cross‐protection against either T. circumcincta, Trichostrongylus axei or Cooperia oncophora . A reciprocal experiment, where sheep were immunized with the equivalent glycoproteins from T. circumcincta, showed that, while they were not protected against homologous challenge, there was some cross‐protection against Haemonchus as measured by a significant reduction in worm egg output.