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Alterations of intralesional and lymph node gene expression and cellular composition induced by IL‐12 administration during leishmaniasis
Author(s) -
Schopf Lisa R.,
Erickson Jamie,
Hayes Lori,
Chung Charles,
Lavigne Liz,
Sypek Joseph
Publication year - 2001
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.2001.00360.x
Subject(s) - lymph node , lymph , immunology , biology , leishmania major , lesion , inflammation , immune system , cutaneous leishmaniasis , leishmaniasis , leishmania , pathology , medicine , parasite hosting , world wide web , computer science
Changes in gene expression and cellular distribution in the lymph node and at the site of infection, the footpad, during Leishmania major infection and/or IL‐12 administration were evaluated. Otherwise susceptible BALB/c mice given IL‐12 are able to resolve infection. Interestingly, iNOS was not induced in the lymph node by IL‐12, yet, nitric oxide is critical in the control of leishmaniasis. However, we observed an increase in iNOS at the lesion site in response to IL‐12. These results reflect the importance of examining the primary site of infection. We observed no changes in inflammation at the lesion site; however, IL‐12 promoted an early inflammatory response in the lymph nodes. IL‐12 administration differentially affected both the local and systemic immune response to invading leishmanial parasites. IL‐12 induced iNOS at the lesion site and an early granulomatous inflammation in the lymph node; therefore, we hypothesize that these are key events responsible for the resolution of disease in BALB/c mice treated with IL‐12.