Premium
Vaccination against coccidiosis: host strain‐dependent evocation of protective and suppressive subsets of murine lymphocytes
Author(s) -
Rose M.elaine,
Hesketh Patricia,
Grencis Richard K.,
Bancroft Allison J.
Publication year - 2000
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.2000.00287.x
Subject(s) - biology , immunology , cd8 , vaccination , population , antibody , immunization , mesenteric lymph nodes , immune system , antigen , cytotoxic t cell , spleen , in vitro , medicine , biochemistry , environmental health
BALB/c mice are normally more resistant than C57BL/6 (B6) mice to infection with Eimeria vermiformis , but these phenotypes can be reversed by oral or parenteral vaccination with a crude antigen prepared from the parasite. Treatment of mice with antibodies specific for CD4 + or CD8 + T cells showed that the increased susceptibility of vaccinated BALB/c mice was associated with the presence of CD4 + T cells. This finding was confirmed when the recipients of CD4 + T cells selected from the mesenteric lymph nodes (MLN) of vaccinated BALB/c mice produced more oocysts after challenge than the recipients of a similar population of cells from sham‐vaccinated mice. The residual population of cells (presumably enriched for CD8 + T cells, ‘CD8 + ’), on the other hand, conferred some protection and, in B6 mice, the findings were reversed. Thus, vaccination induced suppressive or protective CD4 + cells and protective or suppressive ‘CD8 + ’ cells, depending upon the normal resistance/susceptibility phenotype of the host. Examinations of the isotypes (IgG1, IgG2a) of specific serum antibodies, and of the levels of IFN‐γ and IL‐5 cytokines released by MLN cells stimulated ex vivo , did not allow any further characterization of the mechanisms involved .