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The relationship between circulating and intestinal Heligmosomoides polygyrus ‐specific IgG 1 and IgA and resistance to primary infection
Author(s) -
A.W. Ben-Smith,
Faisal Wahid,
David A. Lammas,
Jerzy M. Behnke
Publication year - 1999
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1999.00236.x
Subject(s) - heligmosomoides polygyrus , biology , antibody , immunology , phenotype , immune system , immunoglobulin a , ratón , secretory iga , immunoglobulin g , gene , biochemistry
Specific serum and intestinal immunoglobulin (Ig)G 1 and IgA responses to Heligmosomoides polygyrus were measured in a panel of seven inbred mouse strains which exhibit ‘rapid’ (< 6 weeks (SWR × SJL)F 1 ), ‘fast’ (< 8 weeks, SJL and SWR), ‘intermediate’ (10–20 weeks, NIH and BALB/c) or ‘slow’ (> 25 weeks, C57BL/10 and CBA) resolution of primary infections. Mice with ‘rapid’, ‘fast’ or ‘intermediate’ response phenotypes produced greater serum and intestinal antibody responses than those with ‘slow’ phenotypes. The F 1 hybrids ((SWR × SJL)F 1 ) of two ‘fast’ responder strains showed the earliest antibody response with maximum titres evident within 6 weeks of infection. There was a negative correlation between the serum IgG 1 responses and worm burdens in individual mice within a number of mouse strains, and also between serum IgG 1 and IgA responses and worm burdens in the ‘rapid’ ((SWR × SJL)F 1 ) responder strain. The presence of IgG 1 in the gut was found to be due to local secretion rather than plasma leakage. Using Western immunoblotting, serum IgG 1 from ‘rapid’ and ‘fast’ responder but not ‘slow’ responder mice was found to react with low molecular weight antigens (16–18 kDa) in adult worm excretory/secretory products .