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Humoral responses in mice following vaccination with DNA encoding glutathione S ‐transferase of Fasciola hepatica : effects of mode of vaccination and the cellular compartment of antigen expression
Author(s) -
SMOOKER PETER M.,
STEEPER KELLY R.,
DREW DAMIEN R.,
STRUGNELL RICHARD A.,
SPITHILL TERRY W.
Publication year - 1999
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1999.00235.x
Subject(s) - vaccination , biology , isotype , immunology , dna vaccination , fasciola hepatica , immunogenicity , antigen , virology , immunization , antibody , monoclonal antibody , helminths
The humoral responses in mice following vaccination with DNA constructs encoding Fasciola hepatica glutathione S ‐transferase (GST) have been evaluated. GST47 cDNA was subcloned into two DNA vaccine vectors, VR1012 and VR1020, which direct expression to the cytoplasmic and extracellular compartments, respectively. Expression was confirmed by transfection into COS 7 cells. Groups of mice were vaccinated with these constructs, by either intramuscular injection with the VR1012‐or VR1020‐based constructs, or intradermal vaccination (with a gene gun) with the VR1020‐based construct. Vaccination with the construct designed for secretion resulted in an increased humoral response compared to vaccination with the nonsecretory construct. The level of the total humoral response after vaccination with the secretion construct was not dependent on the route of vaccination. However, the isotype profile of the response differed between the groups; intramuscular vaccination with the construct directing cytoplasmic expression yielded an immuoglobulin (Ig)G 2a dominant (Th1‐type) response, intradermal vaccination with the secretory construct a IgG 1 /IgE dominant (Th2‐type) response, and intramuscular vaccination with the secretory construct a mixed isotype response. These results demonstrate that the immunogenicity of a DNA vaccine based on Fasciola GST, as well as the isotype of the response against GST, is determined by the mode of vaccine administration .