Premium
Antibody response in CD4‐depleted mice after immunization or during early infection with Echinococcus granulosus
Author(s) -
BAZ ADRIANA,
RICHIERI ANA,
PUGLIA ALEJANDRA,
NIETO ALBERTO,
DEMATTEIS SYLVIA
Publication year - 1999
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1999.00212.x
Subject(s) - biology , antigen , echinococcus granulosus , immunology , antibody , pan t antigens , monoclonal antibody , zoology
The aims of this work were to investigate the existence of T‐independent antigens in Echinococcus granulosus protoscoleces and to evaluate the relative contribution of T‐independent stimulation to the overall antibody response in early infection. Mice depleted of CD4 + ‐cells were immunized with protoscolex somatic antigens (PSA) or infected with E. granulosus protoscoleces (PSC) . Results showed that the response of CD4‐depleted immunized mice had the expected characteristics of a T‐independent stimulation and that such T‐independent stimulation was important mainly during primary response. During infection absence of CD4 + ‐cells affected mainly the secretion of all IgG subclasses with the exception of IgG3 and IgM. To carry out a preliminary isolation of PSC T‐independent antigens we prepared a carbohydrate enriched fraction from protoscolex antigens, using a monoclonal antibody specific for the carbohydrate moiety Galα(1,4)Gal highly expressed in PSC. This fraction was mitogenic for naive mouse splenocytes and was recognized by a high percentage of the specific antibodies secreted by CD4‐depleted immunized or infected mice. In summary, these results suggest that E. granulosus protoscoleces contain immunogenic T‐independent antigens. Primary antibody responses to protoscolex somatic antigens and the production of IgM and IgG3 in early infection would be mainly stimulated by a T‐independent mechanism .