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Definition and mapping of epitopes recognized by specific monoclonal antibodies to Schistosoma bovis 28 kDa glutathione S‐transferase: relation with anti‐egg viability immunity
Author(s) -
DA COSTA ALEXANDRA VIANA,
LAFITTE SOPHIA,
FONTAINE JOSETTE,
BOSSUS MARC,
GRASMASSE HÉLÈNE,
CAPRON ANDRÉ,
GRZYCH JEANMARIE
Publication year - 1999
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1999.00196.x
Subject(s) - biology , epitope , monoclonal antibody , antibody , immune system , schistosoma japonicum , immunity , immunology , microbiology and biotechnology , biochemistry , schistosomiasis , helminths
Monoclonal antibodies to the 28kDa glutathione S‐transferase of Schistosoma bovis have been constructed in mice and used to characterize the epitope(s) potentially implied in the induction of anti‐fecundity and anti‐egg viability immune responses. Among the MoAbs produced three were particularly studied: Sb4‐50 (IgG2a) and Sb4‐56 (IgG1) which inhibited Sb28GST activity and Sb4‐10 (IgG1) which did not. The use of overlappting peptides covering the entire amino acid sequence of Sb28GST, allowed us to define the linear epitopes recognized by these anti‐Sb28GST MoAbs. Amino acid residues 202‐211 were recognized by both MoAbs Sb4‐50 and Sb4‐56 and MoAb Sb4‐10 recognized amino acid residues 58‐67. Their capacity to inhibit GST activity suggested binding to the active site or to neighbouring regions, which include the C‐terminal domain (a.a. 190‐211) of the protein. When passively transfered into BALB/c mice MoAbs induced a significant reduction in egg hatching and an increase in immature eggs. Effects on worm burdens were, however, variable and no clear‐cut association between the inhibition of enzyme activity and anti‐fecundity or anti‐viability activities was recorded. Our data indicate that beside the anti‐fecundity and anti‐viability immunity related to the impairment of GST activity, immune response to epitopes located in other regions of the molecule also contribute to the reduction of egg viability .