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DNA immunization by intramuscular injection or gene gun induces specific IgG antibodies against a Schistosoma japonicum 22 kDa antigen, Sj22, when fused to the murine Ig K‐chain secretory leader sequence
Author(s) -
G.J. Waine,
D.R. Mazzer,
Donald P. McManus
Publication year - 1999
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1999.00183.x
Subject(s) - schistosoma japonicum , biology , antigen , gene gun , antibody , dna vaccination , immunization , immunology , virology , microbiology and biotechnology , schistosomiasis , helminths
The 22 kDa tegumental surface membrane‐associated antigen of Schistosoma japonica , Sj22, is of recognized interest in schistosomiasis vaccine research. However, previous attempts to induce antibody responses against Sj22 by DNA immunization have been unsuccessful. In this report we demonstrate that fusing the Sj22 cDNA to the murine immunoglobulin Ig κ‐chain secretory leader sequence results in the generation of antigen‐specific IgG antibodies following DNA immunization. Mice were immunized into the skin with DNA‐coated gold microspheres using a gene‐gun, or into the quadriceps muscle by intramuscular injection. Both methods of delivery generated antigen‐specific IgG antibodies against the 22 kDa schistosome antigen. The use of a secretory leader sequence, such as the murine Ig‐kappa chain used in this study, may facilitate the induction of host antibody responses following DNA immunization with other parasite cDNAs .