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Immunomodulation by hydatid cyst fluid toxin ( Echinococcus granulosus )
Author(s) -
JANSSEN D.,
RUEDA M.C.,
DE RYCKE P.H.,
OSUNA A.
Publication year - 1997
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1997.d01-194.x
Subject(s) - echinococcus granulosus , biology , in vitro , in vivo , cd8 , cd3 , microbiology and biotechnology , immunology , immune system , biochemistry , zoology
Since the experimental infection by hydatid cysts ( Echinococcus granulosus ) in mice causes immunomodulation of the host, the effects of hydatid fluid (HF) and fractions of HF were compared in vitro and in vivo . Fractions of HF were obtained using ammonium sulphate precipitation, chloroform/methanol extraction and thin‐layer chromatography (TLC). HF proved to be toxic to murine peritoneal macrophages in vitro , and when macrophages were incubated with the different fractions of HF, most toxicity was found in a single TLC‐purified fraction with an adjuvant‐like effect on the production of specific antibodies against bovine albumin and human red blood cells in mice. Treatment of mice with the toxin caused a drop in the percentage of peripheral blood lymphocytes. Flow‐cytometric analysis showed that T‐cells from toxin‐treated mice had lower membrane‐CD3, CD4 and CD8 density, and had higher percentages of CD8 + splenocytes and CD4 + thymocytes expressing CD25. The toxin caused a down‐regulation of CD4 and CD8 expression on thymocytes in vitro , that was dependent on the presence of macrophages. The results may attribute to these toxins a role in the host‐parasite relationship of hydatidosis.