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Antibodies against Echinococcus multilocularis alkaline phosphatase as markers for the specific diagnosis and the serological monitoring of Alveolar echinococcosis
Author(s) -
SARCIRON ELISABETH M.,
BRESSONHADNI SOLANGE,
MERCIER MARIETTE,
LAWTON PHILIPPE,
DURANTON CHRISTELLE,
LENYS DANIELE,
PETAVY ANNE F.,
VUITTON DOMINIQUE A.
Publication year - 1997
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1997.d01-183.x
Subject(s) - echinococcus multilocularis , serology , antigen , alkaline phosphatase , echinococcosis , antibody , albendazole , mebendazole , biology , immunology , echinococcus , virology , enzyme , biochemistry , ecology , zoology
The immunological properties of the purified alkaline phosphatase (pAP) of Echinococcus multilocularis metacestodes have been investigated using alveolar echinococcosis (AE) patient sera in ELISA tests. A comparative study was done with EmC‐Ag (crude antigen) and pAP‐Ag (purified antigen). When the parasite purified enzyme pAP was used as antigen, the specificity of the ELISA was markedly increased since it reached 100% without any decrease of its sensitivity (100%). The serologic follow‐up of AE patients was conducted during several months with these two antigens in three categories of patients: cured, stabilized and aggravated. There was a good correlation between clinical and serologic data when the pAP was used as antigen in ELISA tests. The anti‐pAP antibodies titres did change more rapidly than anti‐EmC antibodies titres when a recurrence occurred. Modifications of the anti‐pAP antibodies levels were also observed during the patient’s therapy: mebendazole, albendazole and Isoprinosine ® . These results suggest that pAP‐Ag should be used for the diagnosis and the follow‐up of AE patients.