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Protective immunity induced by vaccination with Onchocerca volvulus tropomyosin in rodents
Author(s) -
TAYLOR MARK J,
JENKINS ROSALIND E.,
BIANCO ALBERT E.
Publication year - 1996
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1996.d01-93.x
Subject(s) - onchocerca volvulus , biology , immunology , vaccination , immunity , onchocerciasis , immune system
A cDNA clone of Onchocerca volvulus , designated MOv14, and encoding 136 amino‐acid residues from the C‐terminus of O. volvulus tropomyosin, was evaluated as a protective immunogen in two complimentary rodent models of onchocerciasis. Vaccination of BALB/c mice with the recombinant fusion of MOv14 coupled to Maltose‐Binding Protein (MBP) induced significant reductions (48–62%) in the recovery of Onchocerca lienalis microfilariae from the skin, compared to control groups immunized with MBP alone. The predominant antibody response generated to MOv14 by vaccination was of IgG1. Following a similar vaccination protocol in Mongolian jirds, two independent experiments demonstrated that 16 weeks after infection with Acanthocheilonemaviteae there was a 46% reduction in the recovery of adult worms in vaccinated animals compared to control groups. Antibodies generated by vaccination recognized a product released during culture of A. viteae infective larvae which migrated at a distinct molecular mass from native tropomyosin from somatic tissues.