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Immunological responses to antigen B from Echinococcus granulosus cyst fluid in hydatid patients
Author(s) -
IOPPOLO S.,
NOTARGIACOMO S.,
PROFUMO E.,
FRANCHI C.,
ORTONA E.,
RIGANO R.,
SIRACUSANO A.
Publication year - 1996
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1996.d01-31.x
Subject(s) - echinococcus granulosus , antigen , biology , isotype , immunology , antibody , echinococcus , peripheral blood mononuclear cell , microbiology and biotechnology , echinococcosis , in vitro , monoclonal antibody , zoology , biochemistry
The immunological reactivity of Echinococcus granulosus antigen B was evaluated in 30 hydatid patients. Antigen B was purified from sheep hydatid cyst fluid by electroelution from a non‐reducing SDS‐PAGE gel (AgB). In ELISA and immunoblotting (IB), determining antibody production in sera from patients with hydatid disease and with other parasitic infections, purified AgB showed higher specificity than a partially purified antigen named pH 5 PPT (100% vs 83% in pH5PPT‐ELISA and 58% in pH 5 PPT‐IB). AgB‐IB achieved higher sensitivity than AgB‐ELISA (80% vs 63%). All AgB‐IB positive sera recognized the 12 kDa subunit. Qualitative AgB‐IB assessment of IgG isotype responses identified IgG4 as the predominant isotype (87%). The other isotypes showed a lower percentage of positive reactions: IgG1, 33%; IgG2, 21%; and IgG3, 17%. PBMC proliferative assay revealed a cellular response to AgB in 100% of patients’ PBMC. These findings confirm antigen B, especially its smallest subunit, as a good diagnostic molecule.