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Humoral immune responses in Gambians to Pfs16, an immunodominant, Plasmodium falciparum integral membrane protein
Author(s) -
BAKER D.A.,
DRAKELEY C.J.,
ONG C.S.L.,
LULAT A.GM.I.,
GREENWOOD B.M.,
TARGETT G.A.T.
Publication year - 1996
Publication title -
parasite immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 75
eISSN - 1365-3024
pISSN - 0141-9838
DOI - 10.1046/j.1365-3024.1996.d01-17.x
Subject(s) - biology , plasmodium falciparum , epitope , peptide , antibody , immune system , gametocyte , virology , humoral immunity , immunology , peptide sequence , malaria , biochemistry , gene
Naturally acquired humoral immune responses to Pfs16, an integral membrane protein expressed in Plasmodium falciparum gametocytes and sporozoites, were investigated in The Gambia. A high prevalence of antibodies to this molecule was detected by peptide ELISA. Ninety‐three per cent of the people taking part in a survey at the end of the rainy season (November) had serum antibodies to one or more synthetic peptides spanning the sequence; 88% reacted with one particular peptide sequence (IMLIILSGIVGFKVK) whereas only one out of ten non‐Gambians (taking anti‐malarial prophylaxis with no history of infection) reacted with the peptide. Epitope mapping with mouse MoAbs has shown that this peptide is located on a part of the molecule differing from immunodominant regions of the molecule identified in a previous study in Papua New Guinea.